Conjugation

Part:BBa_K543032

Designed by: Satoru Shimura   Group: iGEM11_Tokyo_Metropolitan   (2011-10-05)


assay system of killing by conjugation

this part assays conjugation to second round of conjugation. Especially, it is useful to assay killing device by conjugation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 321
    Illegal NheI site found at 367
    Illegal NheI site found at 1028
    Illegal NheI site found at 1074
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 527
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1230
    Illegal NgoMIV site found at 1240
    Illegal NgoMIV site found at 2492
    Illegal NgoMIV site found at 2502
    Illegal AgeI site found at 3327
    Illegal AgeI site found at 3439
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 404
    Illegal BsaI.rc site found at 2154
    Illegal SapI site found at 1132
    Illegal SapI site found at 2394


[edit]
Categories
Parameters
None